Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 2;397(3):592–597. doi: 10.1016/j.bbrc.2010.05.164

Figure 2. GSK-3 activity increases Occludin, E-cadherin and Claudin-1 expression at cell-cell contacts.

Figure 2

(A) The expression of various AJC proteins is shown by immunofluorescence (IF) labeling/confocal microscopy in SK-CO15 cells treated with GSK-3 siRNA. There are no substantial differences between the Mock and Scramble siRNA treated samples, however, incubation with GSK-3 siRNA decreased Occludin and E-cadherin protein levels. There is no change in JAM-A or ZO-1 expression and none of these proteins have altered cellular localization. Bar, 20 μm. (B) Treatment with GSK-3 siRNA decreased Occludin, Claudin-1 and E-cadherin protein levels. There is no change in JAM-A or ZO-1 expression. For A and B representative images of multiple experiments are shown. Bar, 20 μm.