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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Acta Biomater. 2010 Jul 21;7(2):614–624. doi: 10.1016/j.actbio.2010.07.016

Figure 5.

Figure 5

(A) Representative confocal images, 7 slice projections, of encapsulated Lewis rat islets stained using Live/Dead fluorescent kit where green and red fluorescence indicates live or dead cells, respectively. (B) Changes in MTT absorbance readings as a measurement of islet viability following encapsulation with Ba-Alg (open bars) or XAlgPEG (solid bars). Day 0 assessment was performed prior to encapsulation. (C) Glucose stimulated insulin secretion results, measured as insulin release, on Day 1 and Day 5 for Lewis rat islets following encapsulation in Ba-Alg or XAlgPEG (as indicated on horizontal axis). Insulin release following 1 hr incubation in Basal 1 (solid bars), Stimulated (empty bars), and Basal 2 (grey bars) solutions is shown. Data points represent the mean ± SD, for a minimum of three independent measurements at each time point (except the Day 1 for Insulin measurements where Ba-Alg were only duplicates).