Figure 2.

Properties of the dhp1-1 mutation. (A) Construct of the dhp1-1 allele. The ura4⁺ gene was inserted into the EcoRV site of the dhp1⁺ gene to generate Dhp1-1p, which lacks 125 amino acids from the C-terminus of Dhp1p. Only relevant restriction sites are shown. H, HindIII; N, NdeI; RV, EcoRV. (B) Growth rate of the dhp1-1 mutant. Wild-type (JY742, closed squares) or dhp1-1 (MP102, open squares) cells grown at 25°C in YES were cultured at 36°C. (C) Instability of Dhp1-1p at the restrictive temperature. Wild-type or the dhp1-1 cells grown at 25°C in YES were cultured at 36°C. Soluble cell lysates prepared from aliquots were separated by 8.3% SDS–PAGE and subjected to western blotting with anti-Dhp1p antiserum.