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. Author manuscript; available in PMC: 2010 Nov 7.
Published in final edited form as: Traffic. 2006 May;7(5):574–588. doi: 10.1111/j.1600-0854.2006.00410.x

Figure 4. Clathrin is required for progression of Sla2p patches and normal actin dynamics.

Figure 4

(A1–A3). WT ABP1-mRFP SLA2-GFP (SL5185), chc1Δ ABP1-mRFP SLA2-GFP (SL5240) and clc1Δ ABP1-mRFP SLA2-GFP (SL5226) cells were viewed using wide-field microscopy. (B1–B9) Kymographs generated from single pixel wide lines placed through cortical patches from cells described in (A). Images were collected at two-second intervals and viewed up to 4 min. Note different types of patch dynamics in clathrin mutants: (B2 and B6) immobile Sla2p patches without actin assembly; (B3 and B7) Sla2p patches with actin comet tails and repeated patch internalization from the same site; (B4 and B8) Sla2p and Abp1p patches with extended lifetimes that do mature and internalize; (B5 and B9) relatively normal Sla2p lifetimes with extended Abp1p lifetimes. (C and D) Bar graphs showing average patch lifetimes of Sla2p-GFP and Abp1p-mRFP in WT and clathrin mutants for relatively normal internalization events (n = 30). (C) Patch lifetimes of Sla2p in WT and clathrin mutants (B5 and B9) were not significantly different (p > 0.05). (D) Patch lifetimes of Abp1p were slowed almost 2-fold in clathrin mutants (p < 0.001) (B5 and B9) Scale bar = 5 μm.