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. Author manuscript; available in PMC: 2010 Nov 7.
Published in final edited form as: Traffic. 2006 May;7(5):574–588. doi: 10.1111/j.1600-0854.2006.00410.x

Figure 5. Actin comet tails and patches in clathrin mutants contain endocytic membrane.

Figure 5

(A) clc1Δ ABP1-GFP (SL5101) cells stained with 40 μM FM4-64 and incubated for 20 min before viewing using wide-field microscopy. Note patches of Abp1p associated with FM4-64 seen as yellow in the merge. (B) Two examples of time-lapse videos showing concentration of FM4-64 into de novo actin patches (top panel) and actin comet tails (bottom panel) from clc1Δ cells. Note first three frames in the top left corner of B), top panel show a residual disappearing Abp1p patch different than the de novo patch that appears in the middle of frame number 3. Scale bar = 5 μm. Time scales in (B) are in seconds.