Skip to main content
. Author manuscript; available in PMC: 2010 Nov 7.
Published in final edited form as: Traffic. 2006 May;7(5):574–588. doi: 10.1111/j.1600-0854.2006.00410.x

Table 1.

Saccharomyces cerevisiae strains

Strain Genotypea
HR1965 MATa his4 leu2 ura3 his4 lys2 bar1 sla2Δ::LEU2d
HR3399 MATa leu2 ura3 his3 sla2Δ::LEU2 sla2Δ376-573::TRP1d
SL1463 MATα leu2 ura3-52 trp1 his3-Δ200
SL1628 MATa leu2 ura3-52 trp1 his3-Δ200 clc1Δ::HIS3 YEp24-CHC1 [2μ, URA3, CHC1]
SL1629 MATa leu2 ura3-52 trp1 his3-Δ200 YEp24-CHC1
SL1927 MATα leu2 ura3-52 ade6 chc1Δ::LEU2 pKH2 [2μ, URA3, CLC1]
SL4409 MATa leu2-3,112 ura3-52 his3-Δ200 trp1-901 gal4Δ ade2 gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ chc1Δ::TRP1b
SL4410 MATa leu2-3,112 ura3-52 his3-Δ200 trp1-901 gal4Δ ade2 gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ clc1Δ::TRP1b
SL4781 MATα leu2 ura3 his3-Δ200 trp1 sla2Δ::LEU2 p111-SLA2-GFP [CEN, LEU2, TRP1, SLA2-GFP]
SL4782 MATa leu2 ura3 his3-Δ200 trp1 clc1Δ::KanMX6 sla2Δ::LEU2 p111-SLA2-GFP [CEN, LEU2, TRP1, SLA2-GFP]
SL5090 MATα leu2 ura3-52 trp1 his3-Δ200 clc1Δ::HIS3 ABP1-mRFP::KanMX6 pTMN17 [CEN, TRP1, GFP-CLC1]
SL5101 MATa leu2 ura3 his3 ABP1-GFP::HIS3 clc1Δ::KanMX6 pRS426 [2μ, URA3]c
SL5121 MATα leu2-3,112 ura3-52 trp1 his3-Δ200 clc1Δ::HIS3 SLA2-mRFP::KanMX6 pTMN17 [CEN, TRP1, GFP-CLC1]
SL5185 MATα leu2 ura3 trp1 his3-Δ200 sla2Δ::LEU2 ABP1-mRFP::KanMX6 p111-Sla2-GFP [CEN, LEU2, TRP1, SLA2-GFP]
SL5186 MATα leu2 ura3 trp1 his3-Δ200 sla2Δ::LEU2 ABP1-mRFP::KanMX6 pTMN52 [CEN, LEU2, sla2Δcc-GFP::TRP1]
SL5198 MATa leu2 ura3 his3 clc1Δ::HIS3 sla2Δ::HIS3 sla2Δcc::TRP1 ABP1-mRFP::KanMX6 pTMN37 [CEN, URA3, GFP-CLC1]d
SL5226 MATa leu2 ura3 his3-Δ200 trp1 chc1Δ::LEU2 sla2Δ::LEU2 ABP1-mRFP::KanMX6 p111-Sla2-GFP [CEN, LEU2, SLA2-GFP::TRP1]
SL5240 MATa leu2 ura3 his3-Δ200 trp1 clc1Δ::HIS3 sla2Δ::LEU2 ABP1-mRFP::KanMX6 p111-Sla2-GFP [CEN, LEU2, SLA2-GFP::TRP1]
SL5317 MATα leu2 ura3-52 trp1 his3-Δ200 clc1Δ::HIS3 SLA2-mRFP::KanMX6 SLA1-GFP::TRP1
SL5318 MATα leu2 ura3-52 trp1 his3-Δ200 SLA2-mRFP::KanMX6 SLA1-GFP::TRP1
YPJ96-4 MATa leu2-3,112 ura3-52 his3-Δ200 trp1-901 gal4Δ ade2 gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZb
a

Strains were generated in our laboratory, except where indicated. Several strains were derived from crosses or transformations into strains from other sources.

b

Derived from YPJ96-4 (64).

c

SL5101 was generated by crossing in an ABP1-GFP::HIS3 strain from D. Drubin (40).

d

HR1965 and HR3399 are described in Wesp et al., 1997 (41). SL5198 was generated by a cross to the sla2Δcc mutant from Wesp et al., 1997 (41).