Figure 1.

Stretched shape of lagging kinetochores is lost after severing microtubules on one side. (A) A scheme of the experiment. Pulling forces of microtubules emanating from opposite spindle poles induce lateral stretching of merotelically attached kinetochores. After severing microtubules attached to a stretched merotelic kinetochore on one side, the kinetochore should resume normal globular shape. (B) pcs1Δ mutant cells expressing mCherry-tubulin and Nuf2-GFP to label spindle microtubules and kinetochores respectively, were imaged before and after laser ablation. The laser ablated area is indicated by a yellow dot. In the control experiment on the right, laser was focused outside the spindle. (C) The loss of stretched shape of lagging kinetochores after laser ablation was quantified in pcs1Δ and clr4Δ mutant cells.