Figure 3.
Inactivation of Cdc2 inhibits phosphorylation of Mde4. (A) nda3-KM311 cells expressing Mde4-GFP (JG15033) were grown at permissive temperature of 34°C (cycling cells) or arrested at non-permissive temperature of 18°C for 8 hours and subsequently released to permissive temperature. Samples were taken at the indicated time-points after the release and Mde4-GFP was analyzed by western blot analysis. (B) nda3-KM311 cdc2-as cells expressing Mde4-GFP (JG15356) were grown at permissive temperature of 34°C (cycling cells) or arrested at non-permissive temperature of 18°C for 8 hours. Cdc2-as was subsequently inactivated by adding 5 μM 1-NM-PP1. Samples were taken at the indicated time-points after adding 5 μM 1-NM-PP1 and Mde4-GFP was analyzed by western blot analysis.