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. Author manuscript; available in PMC: 2010 Nov 7.
Published in final edited form as: Cell Cycle. 2010 Oct 3;9(19):3997–4004. doi: 10.4161/cc.9.19.13233

Figure 4.

Figure 4

Hypo-phosphorylated Mde4 localizes to metaphase spindles and kinetochores. (A) A scheme of experimental procedure. Hypo-phosphorylated Mde4 and hyper-phosphorylated Mde4 indicate experimental stages, which are referred to in other parts of the Figure. (B) nda3-KM311 cdc2-as cells expressing Mde4-GFP (JG15356) were harvested at stages indicated in the (A), fixed and stained with antibodies against tubulin and GFP. Nuclei were visualized by Hoechst staining. (C) Untagged nda3-KM311 cells (JG12013) or nda3-KM311 cdc2-as cells expressing Mde4-GFP (JG15356) were harvested at stages indicated in the (A). Chromatin binding of the Mde4-GFP was analyzed by chromatin immunoprecipitation followed by quantitative PCR using oligonucleotide primers specific for the centromeric central region (cnt2), innermost centromeric repeats (imr2), outer centromere (dgII) and chromosome arm (top1).

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