Table 2.
Genes Encoding Members of the TGFß Superfamily and their Associated Signaling Proteins which are Differentially Expressed During Murine Orofacial Ontogenesis.
| Probe Set ID | Gene | 1Fold Change GD-13 vs. GD-12 | 1Fold Change GD-14 vs. GD-13 |
|---|---|---|---|
| 101918_at | TGF beta1 | -1.12 | 6.50 |
| 103209_at | Latent transforming growth factor beta binding protein 1 | 1.10 | 2.14 |
| 161307_f_at | Calpain 10 | 1.05 | -5.04 |
| 92237_at | Retinoid X receptor gamma | -1.05 | 2.10 |
| 92695_at | Frequently rearranged in advanced T-cell lymphomas (Frat-1) (Wnt signaling; cell growth & maintenance) | -2.20 | -1.20 |
| 97769_at | Prostaglandin F receptor | 2.83 | 1.07 |
| 93534_at | Decorin | 3.33 | 1.62 |
| 94179_at | Dachshund-1 | 1.35 | -2.30 |
| 101815_at | BMP10 | -2.52 | 1.63 |
| 92485_at | BMP15 or GDF-9B | 1.41 | -2.70 |
| 96920_at | Protease, serine, 11 (Prss11) (Igf binding) [Negative regulation of BMP signaling] | 2.20 | 2.25 |
| 99865_at | BMP receptor, type II | -2.14 | 1.18 |
| 93452_at | Cerberus 1 | 3.18 | -2.14 |
| 102266_at | Inhibin alpha | 2.25 | -1.55 |
| 97734_at | Thrombopoietin | -2.41 | 1.18 |
| 160109_at | Sox-4 | -2.05 | -1.023 |
| 102994_at | Signal transducer and activator of transcription 4 (Stat4) | 2.56 | -2.00 |
| 100278_at | Cyclin-dependent kinase inhibitor p27kip1 (Cdkn1b) | -1.91 | -1.15 |
| 98067_at | Cyclin-dependent kinase inhibitor 1A (p21) | 1.78 | 2.20 |
| 101900_at | Cyclin dependent kinase inhibitor 2B (Cdkn2b or p15) | 3.18 | 1.18 |
| 94881_at | Cyclin-dependent kinase inhibitor 1A (p21) | 1.90 | 2.00 |
| 94305_at | Alpha 1 type I procollagen | 1.80 | 3.03 |
| 161716_at | Fos (FBJ osteosarcoma oncogene) | -3.33 | 1.50 |
| 92739_at | Involucrin (keratinocyte differentiation) | 1.55 | -2.10 |
| 102218_at | Interleukin 6 | 2.50 | -1.45 |
| 161509_at | MMP2 (gelatinase A) | -2.41 | 2.35 |
| 99957_at | MMP9 (gelatinase B) | 6.96 | 1.12 |
Triplicate gene expression data sets from orofacial tissue from each of GD-12, GD-13 and GD-14 embryos were filtered and the average fold change for each gene was calculated from three different comparisons of the gene expression patterns of GD-13 vs. GD-12 and GD-14 vs. GD-13 orofacial tissue. Only those genes which demonstrated a statistically significant (p<0.005) increase or decrease in expression of at least two-fold in all three biological replicates for either the GD-13 vs. GD-12 expression comparison or the GD-14 vs. GD-13 expression comparison were included in this table. Note that GD-13 vs. GD-12 means that expression on gestation day 12 was utilized as the baseline, and that GD-14 vs. GD-13 means that expression on gestation day 13 was utilized as the baseline. Therefore, negative numbers indicate a decrease in expression, whereas positive numbers indicate an increase in expression.