Table 1.
CD signal (nm) | Assignment | T m′ °C (WT) | T m′ °C (dgd1) |
---|---|---|---|
685–730 | Ψ-type | 54 ± 2 | 48 ± 1 |
685–671 | Ψ-type | 54 ± 1 | 49 ± 1 |
505–550 | Ψ-type | 56 ± 1 | 51 ± 1 |
610–650 | Excitonic (Chl b, LHCII) | 61 ± 2 | 58 ± 2 |
448–459 | Excitonic (Chl a) | 59 ± 2 | 54 ± 1 |
448–438 | Excitonic (Chl a) | 57 ± 1 | 50 ± 1 |
The membranes were thermostated for 10 min at different temperatures in the range between 5 and 80°C before recording the CD spectra at the given temperature; the amplitudes for the individual bands were calculated from the difference in the intensity at specific wavelengths (see also the text). T m is defined as the temperature at which the intensity of the CD band is decreased to 50% of its value at 25°C. The values for T m and their standard errors are determined from five independent experiments