Table II.
Thermodynamic and Kinetic Folding Data Derived From Chemical Denaturation of HSBD at 298 K
| Ionic strength (M) |
 (kcal mol−1 M−1) |
[D]50% (M) |
 (kcal/mol) |
mTS-D (kcal mol−1 M−1) | mTS-N (kcal mol−1 M−1) |
 (kcal mol−1 M−1) |
kf (s−1) | ku (s−1) |
 (kcal/mol) |
|---|---|---|---|---|---|---|---|---|---|
| 0.5 | 0.98 ± 0.02 | 1.38 ± 0.01 | 1.35 ± 0.03 | 0.56 ± 0.03 | 0.32 ± 0.02 | 0.88 ± 0.04 | 8800 ± 200 | 900 ± 100 | 1.4 ± 0.1 |
| 1.0 | 1.12 ± 0.02 | 1.68 ± 0.01 | 1.90 ± 0.03 | 0.59 ± 0.03 | 0.44 ± 0.04 | 1.03 ± 0.05 | 15,800 ± 200 | 430 ± 90 | 2.1 ± 0.1 |
Errors are propagated standard errors.