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. 2010 Aug 20;285(46):35267–35273. doi: 10.1074/jbc.M110.161208

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Flow cytometric analysis of lymphoid cell development of Rad9^+/+ and Rad9^−/− mice. Cells isolated from the bone marrow (A) and spleen (B) of 6–8-week-old mice were stained with the antibodies indicated in each figure. Numbers show relative percentages of cells within indicated gates. These analyses are averages of three pairs of Rad9^+/+ and Rad9^−/− mice. A, in the bone marrow (BM), top row, staining for pro/pre-B (IgM⁻B220⁺), immature (IgM⁺B220^lo), and mature recirculating (IgM⁺B220^hi) B cells; bottom row, staining for pro-B cells (IgM⁻B220⁺CD43⁺) and pre-B cells (IgM⁻B220⁺CD43⁻). B, in the spleen, top row, staining for immature (IgM⁺B220^lo) and mature (IgM⁺B220^hi) B cells; second row, staining for follicular B cells (IgM^loIgD^hi), transitional type-2 B cells (IgM^hiIgD^hi), and marginal zone/transitional type-1 B cells (IgM^hiIgD^lo); third row, staining for transitional type-1 B cells (CD23⁻IgM^hiCD21^lo) and marginal zone B cells (CD23⁻IgM^hiCD21^hi).