Skip to main content
. 2010 Sep 9;285(46):35330–35339. doi: 10.1074/jbc.M110.136259

FIGURE 1.

FIGURE 1.

Effects of ER stress on IL-8 and CHOP production in human intestinal epithelial cells. A, HCT-8, HT29, or HCT-116 cells were treated with TG for 1 h, and each mRNA was measured using RT-real-time PCR. The boxed panel below the graph indicates representative results of three independent experiments using RT-conventional PCR. B, HCT-8 cells transfected with IL-8 reporter plasmid were treated with 0.1 μm TG for 12 h. C, HCT-8 cells were treated with 0.1 μm TG or 1 μg/ml tunicamycin, and each mRNA was measured using RT-real-time PCR. The boxed panel below the graph indicates representative results of three independent experiments using RT-conventional PCR. D, HCT-8 cells transfected with CHOP reporter plasmid were treated with TG for 12 h. Groups with an asterisk are significantly different (p < 0.05) from the vehicle control group. Error bars, S.D.