FIGURE 2.
Mgl-1 associates with RanBPM both in vitro and in vivo. A, pull-down assay. Recombinant GST or GST-Mgl-1 fusion proteins purified by glutathione-SepharoseTM 4B were incubated with RanBPM cell lysates, and the bound proteins were analyzed by immunoblotting with an anti-RanBPM antibody. B, Myc-tagged full-length Mgl-1 and Flag-tagged full-length RanBPM were co-transfected into 293T cells, and immunoprecipitation was performed by using either an anti-Myc or an anti-Flag antibody, followed by Western blotting with anti-Myc and anti-Flag antibodies.