FIGURE 6.

Synthetic peptide P326TAT blocks hyperoxia-induced increase in protein tyrosine nitration. PAEC were exposed to normoxia or hyperoxia (95% oxygen) in the presence and absence of P326TAT and PlwTAT at final concentration 20 μm for 24 h. Then, protein tyrosine nitration was assayed as described under “Experimental Procedures.” A is a representative immunoblot from three experiments. B is a bar graph showing the changes in tyrosine nitration of proteins at 250, 100, 75, and 60 kDa. Results are expressed as mean ± S.D.; n = 3 experiments. *, p < 0.05 versus normoxia group.