FIGURE 8.

Hyperoxia increases eNOS-β-actin association, eNOS activity, and protein tyrosine nitration in mouse lungs. Male C57BL/6 mice were exposed to 85% oxygen for 5 days, then eNOS-β-actin association (A and B), eNOS activity (C), and protein nitrotyrosine (D and E) were assayed in the lung homogenates as described under “Experimental Procedures.” A and D are representative immunoblots from 10 experiments. B is a bar graph showing the changes in the ratio of eNOS to β-actin in the immunoprecipitates. E is a bar graph showing the changes in protein tyrosine nitration. Results are expressed as mean ± S.D.; n = 10 experiments. *, p < 0.05 versus normoxia control.