FIGURE 8.

Relative amounts of Gα subunit mRNAs. CD4⁺ and CD8⁺ T cell subsets were purified by cell sorting from peripheral blood and analyzed by semiquantitative real-time RT-PCR for the expression of mRNAs for the indicated G protein α-subunits and GAPDH for determining ΔC_T values. A, -fold differences in mRNAs in CD4⁺ T cell subsets. Within each subset of cells, the -fold differences in expression were calculated versus the mRNA with the lowest expression (value set to 1), as shown above the positions for Gα_o in the CD62L⁺CD45RO⁻ cells and Gα_i1 in the other subsets. B, -fold differences in mRNAs in CD8⁺ T cell subsets. Within the CD62L⁺CD45RO⁻ subset, the -fold differences were calculated versus the mRNA for Gα_i1. For the other subsets, because the mRNA for Gα_i1 was not detectable (ND), the values for Gα_i3 and Gα_o were normalized to the value for Gα_i2, which was set in each case equal to the Gα_i2 value in the CD62L⁺CD45RO⁻ subset. C, Gα_o mRNA as a percentage of Gα_i2 mRNA in CD4⁺ T cell subsets. D, Gα_o mRNA as a percentage of Gα_i2 mRNA in CD8⁺ T cell subsets. Data shown are for one donor and are representative of three donors for each group of CD4⁺ or CD8⁺ T cell subsets.