FIGURE 5.

Thioflavin T analysis of inhibition of Aβ fibril formation by purified MBP peptides. Aβ40DI was treated with hexafluoroisopropanol, resuspended to a concentration of 2.5 mm in DMSO, and then diluted to a concentration of 12.5 μm in PBS in the absence (□) or presence of 1.56 μm MBP peptides. Aβ40DI + MBP1, ●; Aβ40DI + MBP2, ♦; Aβ40DI + MBP3, ■; Aβ40DI + MBP4, ▴). At specific time points aliquots were collected from each sample and assayed by thioflavin T binding and fluorescence to determine fibrillar assembly. MBP1 is as capable as intact MBP at inhibiting Aβ40DI fibrillogenesis. The data shown are the mean ± S.D. of triplicate samples. A.U., absorbance units.