FIGURE 6.

MBP1 binds to and inhibits Aβ peptide fibril formation. A, interaction of Aβ42WT and Aβ40DI with MBP or MBP1 was analyzed by solid phase binding assay. The data shown are the mean ± S.D. of triplicate determinations. B–D, 100 μm Aβ42WT was incubated for 24 h in the absence or presence of 12.5 μm MBP1 or MBP. Samples were imaged by TEM (scale bars, 100 nm). E–J, 12.5 μm Aβ40DI was incubated for 6 h in the absence or presence of 1.56 μm MBP1 or MBP. Samples were imaged by TEM (scale bars in E–G, 100 nm) and by AFM (scale bars in H–J, 100 nm). Both MBP1 and MBP are capable of binding and inhibiting Aβ42WT or Aβ40DI fibrillogenesis.