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. 2010 Sep 8;285(46):35654–35664. doi: 10.1074/jbc.M110.128959

FIGURE 7.

FIGURE 7.

Tgfβ2 promotes Smad2/3 binding, histone H3 acetylation, and RNA Pol II localization to the Arf locus in MEFs. A, a schematic diagram shows Distal, Proximal. and Intron regions of Arf locus used in ChIP assays. B, shown is quantitative analysis of representative ChIP assays of using wild type MEFs exposed to vehicle (V) or Tgfβ2 (T2) for 1.5 h (a–c) or 24 h (c) after serum deprivation. A ChIP assay was carried out using antibodies specific to Smad2/3 (a), histone H3 acetylated at lysines 9 and 14 (H3-Ac) (b), and RNA polymerase II (RNA Pol II) (c). Immunoprecipitated DNA and input DNA were amplified with primers for genomic regions indicated in A or for the Tgfβ2 non-responsive gene Necdin. p values are as follows: a, 0.081 (*), 0.001 (#), 0.55 (@); b, 0.045 (*), 0.183 (#), 0.738 (@); (c) 0.059 (*) for Tgfβ2 versus corresponding vehicle.