Scheme of the PN promoter from Azoarcus sp. CIB. The nucleotide sequence from positions −174 to +79 is presented. The transcription start site (+1) and the inferred −10 and −35 boxes of the PN promoter are indicated. The ribosome-binding site (RBS) and the ATG start codon of the bzdN gene are also shown in italic and boldface type, respectively. The BzdR-binding regions I–III (operators) are boxed. The palindromic regions containing the TGCA repeats within the operator regions are marked with arrows below the sequence. The FNR (AcpR)-binding site (FNR) is boxed with broken lines, and the inverted repeats of the consensus FNR-binding sequence are marked with convergent broken arrows above the sequence. The ends of the mI (−61 to +79), mII (−112 to −38), and mIII (−174 to −101) DNA probes are indicated as pentagons, triangles, and rectangles, respectively.