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Two-hybrid interaction of Maf1 domains. pACT2-Maf1-A(1–12), pACT2-Maf1-A(1–16), pACT2-Maf1-A(1–23), pACT2-Maf1-A(1–34), pACT2-Maf1-A(1–39), and pACT2-Maf1-A(1–42) plasmids were transformed individually together with pAS2 (control plasmid) or pAS2-Maf1-BC(196–349) plasmids into yeast strain Y190. Transformants were assayed for β-galactosidase expression using an overlay plate assay.
