FIGURE 4.
Regulation of Pol III transcription and growth is impaired by the K35E mutation in region encoding A domain and restored by second-site suppressor mutations in BC domain of MAF1. maf1-Δ, maf1-K35E, maf1-K35E/D250E, and maf1-K35E/V260D/N344I mutants and isogenic wild type strain YPH500 (WT) were used. A, cells were grown to exponential phase in glucose medium (YPD) at 30 °C, then transferred to glycerol medium (YPGly) and incubated at 37 °C for 1.5 h. Total RNA isolated from cells was tested by Northern blotting with pre-tRNALeu and tRNAPhe probes. B, 10-fold serial dilutions of cells grown to exponential phase in glucose medium were plated on glucose medium (YPD) and incubated at 30 °C or on glycerol medium (YPGly) and incubated at 37 °C for 2–3 days.