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. 2010 Sep 3;285(46):35719–35727. doi: 10.1074/jbc.M110.125286

FIGURE 5.

FIGURE 5.

Two-hybrid interaction of Maf1 domains is impaired by primary mutations in these domains and restored by second-site suppressor mutations. A, pACT2-Maf1-A(1–42) plasmid and its mutated version were transformed individually with pAS2-Maf1-BC(196–349) plasmid or its mutated versions in yeast strain Y190. Transformants were assayed for β-galactosidase expression. Presented profile compares following two-hybrid interactions: wild type A and BC domains of Maf1 (1); mutated A domain K35E with wild type BC domain (2); mutated A domain K35E with four versions of mutated BC domain: D250E (3), V260D/N344I (4), V260D (5), N344I (6); and wild type A domain with mutated BC domain containing single D250E, V260D, or N344I and double N344I/V260D mutations (7-10). B, scheme of Maf1 domain interaction is shown.