FIGURE 6.

Mutations altering interaction of Maf1 domains affect kinetics of Maf1 dephosphorylation upon transfer of yeast from glucose to medium with nonfermentable carbon source. maf1-K35E, maf1-K35E/D250E, and maf1-K35E/V260D/N344I mutant strains and isogenic wild type strain YPH500 (WT) were grown to exponential phase in YPD glucose medium (Exp), then transferred to glycerol YPGly medium, incubated at 37 °C, and harvested as indicated. Protein extracts of lysed cells were analyzed by Western blotting with polyclonal anti-Maf1 antibodies.