FIGURE 5.

Effects of SQSTM1 and HDAC6 on proinflammatory gene expression induced by TLR stimulation. A, expression levels of Sqstm1 and Hdac6 in parental RAW264.7 cells. Cells were stimulated with 1 μg/ml Pam₃CSK₄, 100 ng/ml LPS, or 10 μm ODN 1826 for the indicated periods. The expression levels of Sqstm1 and Hdac6 in cells were determined by qRT-PCR. Each value is expressed as the mean ± S.D. (error bars) of three independent experiments by taking the control group as 1 (*, p < 0.01; †, p < 0.05, for comparison with the control groups). B, RAW264.7 cells transfected with siRNA against Sqstm1 or Hdac6 or with control siRNA were stimulated with 1 μg/ml Pam₃CSK₄, 100 ng/ml LPS, or 10 μm ODN 1826 for 6 h. The expression levels of Sqstm1, Hdac6, Il6, Nos2, Tnf, and Nfkbiz in cells were determined by qRT-PCR. Each value is expressed as the mean ± S.D. of three independent experiments by taking the control group as 1 (*, p < 0.01; †, p < 0.05, for comparison with the control groups).