FIGURE 6.

Effects of SQSTM1 and HDAC6 on signaling events activated by LPS. Immunoblot analysis of parental RAW264.7 cells transfected with siRNA against Sqstm1 or Hdac6. Cells were stimulated with 100 ng/ml LPS for the indicated periods. Whole cell lysates were analyzed by immunoblotting with antibodies to phosphorylated (p-) JNK, p38, ERK, and Akt and IκBα. Total JNK, p38, ERK, Akt, SQSTM1, and HDAC6 served as loading controls. Results are representative of three independent experiments. Densitometric quantification was performed on all of the immunoblot bands obtained by three independent experiments. Each value of phospho-p38, phospho-JNK, phospho-ERK, phospho-Akt (Ser⁴⁷³), and phospho-Akt (Thr³⁰⁸) was normalized to each level of total p38, total JNK, total ERK, total Akt, and total Akt, respectively. Results were expressed as the mean ± S.E. (error bars) of three independent experiments and as -fold increase by taking the control values (control siRNA, 0 min) as 1. *, p < 0.05, for comparison with the control group.