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Tat2 is present at the plasma membrane longer in the absence of Pdr5 and Yor1. Wild-type and pdr5Δ yor1 strains were transformed with pRS316-Cup1-Tat2-GFP-3×HA (pSR74), and transformants were grown overnight in CSM −URA media. These cultures were shifted to CSM −URA media containing 4 μg/ml tryptophan, grown for 6 h, and visualized by Nomarski optics and fluorescence microscopy.
