TABLE 1.
Strains and plasmids used in this study
| Strain | Genotype | Source/Ref. |
|---|---|---|
| SEY6210 | MATα leu2-3,-112 ura3-52 lys2-801 trp1-901 his3-200 suc2-9 Mel- | Scott Emr |
| SEY6210 pdr5Δ yor1 | SEY6210 pdr5-Δ1::hisG yor1::hisG | Moye-Rowley laboratory collection |
| SEY6210 rsb1Δ | SEY6210 rsb1::natMX4 | 9 |
| SRY5 | SEY6210 pdr5-Δ1::hisG yor1::hisG rsb1:natMX4 | This study |
| SRY149 | SEY6210 bul1::kanMX4 | This study |
| SRY150 | SEY6210 rsb1::natMX4 bul1::kanMX4 | This study |
| SRY156 | SEY6210 pdr5-Δ1::hisG yor1::hisG bul1::kanMX4 | This study |
| Plasmid | Plasmid description | Source/Ref. |
|---|---|---|
| pSLP8 | pRS316-RSB1–3×HA | 9 |
| pSR74 | pRS316-CUP1-TAT2-GFP-3×HA | This study |
| pSR86 | pRS316-RSB1Δ335–382-3×HA | This study |
| pSR87 | pRS316-RSB1Δ360–383-3×HA | This study |