FIGURE 4.
Mutation of Cα subunit influences intracellular holoenzyme dynamics. BRET analyses were carried out as detailed in the legend to Fig. 1. A, plasmids coding for GFP2-Cα and GFP2-CαL283R were co-transfected with constructs expressing either RIIα-Rluc or RIIαS99A-Rluc. B, as in A, combined with either RIIβ-Rluc or RIIβS114A-Rluc constructs. Depicted are original BRET values (mean ± S.E.) obtained from at least three independent repeats (n = 6 wells; **, p < 0.01; *, p < 0.05). +, indicates treatment with forskolin/IBMX; −, indicates mock treatment. The dotted line represents the mean background value.