FIGURE 6.

CD44 activates EGFR to regulate MMP-9-enhanced cell migration. A, dose-dependent inhibition of MMP-9-mediated cell migration using an EGFR inhibitor (AG1478). COS-1 cells transfected with vector or MMP-9 cDNAs were treated with different concentrations of AG1478 for 30 min before being subjected to a transwell migration assay; p values reflect comparison with MMP-9-transfected cells: *, p < 0.05. B, activation of EGFR downstream effectors in COS-1 cells transfected with MMP-9 cDNAs, but not in CD44-silenced COS-1 cells. Cell lysates of transfected COS-1 cells were prepared and subjected to Western blot analysis using antibodies against pERK1/2, ERK1/2, pAKT, AKT, pFAK, FAK, pEGFR, EGFR, and α/β-tubulin antibodies.