FIGURE 6.

Insl6 transgenic mice exhibit improved muscle regeneration and enhanced satellite cell activation following CTX injury. A, representative images of H&E-stained TA muscle sections at day 1 and day 7 post-injury. Muscle regeneration was quantified in histological sections by counting nascent myofibers and measuring the cross-sectional area of myofibers at day 7. Data are expressed as a percentage relative to the mean value of WT mouse at 7 days post-injury. B, creatine kinase levels were measured in serum at day 7. TUNEL staining was performed on histological sections from the 7 day time point. C, representative images of TA muscle sections stained with BrdU or Ncam1 (red), with a DAPI counter stain (blue) at 2 days post injury (n = 6). Quantitative data are presented as the percentage of BrdU- or Ncam1-positive cells relative to the total number of DAPI-positive cells. The results are presented as the mean ± S.E. **, p < 0.01; *, p < 0.05.