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. 2010 Sep 8;285(46):36129–36137. doi: 10.1074/jbc.M110.152777

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Acetylated survivin forms homodimers while deacetylated survivin forms monomers and binds to CRM1. A, HeLa cells expressing MYC-tagged survivin129K or -129E were treated with or without the CRM1 inhibitor, leptomycin (LMB). Cells were fixed and immunostained with a MYC antibody and stained with DAPI. Images were taken using a Nikon Confocal microscope. Scale bars, 20 μm. B, lysates from HEK293T cells transfected with cDNAs encoding MYC- or Flag-tagged survivin 129K or 129E were immunoprecipitated with Flag antibody then immunoblotted with MYC or Flag antibody. C, acceptor photobleaching FRET analysis performed on HeLa cells cotransfected with cDNAs encoding MYC-survivin129E and Flag-survivin129E or MYC-survivin129K and Flag-survivin129K. D, lysates from Coriell lymphoblast cell lines homozygous for the A/A (129K) or G/G (129E) alleles at the 129 position were immunoprecipitated with CRM1 antibody then immunoblotted with survivin antibody. LC IgG, light chain immunoglobulin.