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. 2010 Sep 8;285(46):36129–36137. doi: 10.1074/jbc.M110.152777

FIGURE 5.

FIGURE 5.

Nuclear survivin129K binds to the transactivation domain of STAT3. A, HeLa cells treated with or without IL-6, were fixed and immunostained with an antibody to survivin or STAT3, and stained with DAPI. Images were taken with a Nikon Confocal microscope. Bars, 20 μm. B, lysates from HeLa or MCF7 cells treated with or without IL-6 were immunoprecipitated with an antibody to STAT3 then immunoblotted with an antibody to survivin or STAT3. IP, immunoprecipitation; IB, immunoblot. C, HEK293T cells were cotransfected with cDNAs encoding MYC-tagged deletion mutants of STAT3 together with GFP-tagged survivin. Lysates were immunoprecipitated with a MYC antibody then immunoblotted with a GFP antibody. D, lysates from Coriell lymphoblast cell lines homozygous for the A/A (129K) or G/G (129E) alleles at the 129 position were immunoprecipitated with survivin or an IgG control then immunoblotted with anti-STAT3 or anti-survivin. E, HeLa cells expressing MYC-survivin 129K or 129E were fixed and immunostained with anti-MYC and anti-STAT3. Images were taken with a Nikon Confocal microscope. Scale bars, 20 μm.