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. 2010 Sep 13;285(46):36179–36187. doi: 10.1074/jbc.M110.128488

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — P2X7-specific calcium response in transfected cells. A–C, P2X7 receptor function in transfected cell lines was studied by detecting the cytoplasmic free calcium ([Ca²⁺]_i) with the specific agonist BzATP using a Hitachi F-4500 spectrophotometer with fura-2/AM. BzATP was added at the time points indicated to a final concentration of 100 μm () or 300 μm (▴). In blocking experiments, KN62 was preincubated with cells for 0.5 h before [Ca²⁺]_i analysis. [Ca²⁺]_i (nm) is shown on the y axis. The time scale of 100 s is indicated. D, calcium response influx is expressed as a percentage of the maximal response to 100 μm BzATP in K562-W cells, which was defined as 100% response. The curves shown were calculated by nonlinear regression analysis. Data represent the typical results from at least three independent experiments.

Inline graphic — P2X7-specific calcium response in transfected cells. A–C, P2X7 receptor function in transfected cell lines was studied by detecting the cytoplasmic free calcium ([Ca²⁺]_i) with the specific agonist BzATP using a Hitachi F-4500 spectrophotometer with fura-2/AM. BzATP was added at the time points indicated to a final concentration of 100 μm () or 300 μm (▴). In blocking experiments, KN62 was preincubated with cells for 0.5 h before [Ca²⁺]_i analysis. [Ca²⁺]_i (nm) is shown on the y axis. The time scale of 100 s is indicated. D, calcium response influx is expressed as a percentage of the maximal response to 100 μm BzATP in K562-W cells, which was defined as 100% response. The curves shown were calculated by nonlinear regression analysis. Data represent the typical results from at least three independent experiments.