FIGURE 3.

P2X7-specific activation of MAPK signal pathways in transfected cells. A, the P2X7-mediated activation of ERK, JNK, and p38 MAPK signal pathways in K562-V, K562-W, and K562-M cells was studied by Western blotting 5 min post-stimulation by 100 or 300 μm BzATP as described under “Experimental Procedures.” Antibodies recognizing their native proteins were used as internal controls. B, the dose-dependent effects of agonist on the activation of ERK1/2 in K562-W and K562-M cells were studied using different final concentrations of BzATP as indicated. C, cells were pretreatment with KN62 before stimulation by BzATP. D, cells were pretreatment with the MEK inhibitor U0126 before stimulation by BzATP. The relative phosphorylation intensities were quantified using AlphaEaseFC 4.0 software.