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. 2010 Sep 13;285(46):36245–36254. doi: 10.1074/jbc.M110.126003

FIGURE 1.

FIGURE 1.

Ligand-induced Notch signaling in Slc35c1−/− MEFs. A, PCR genotyping of Slc35c1−/− and Slc35c1+/+ MEFs was performed as described under “Experimental Procedures.” B, Slc35c1−/− (black line; C1−/−) and Slc35c1+/+ (gray line; C1+/+) MEFs were analyzed by flow cytometry for cell surface glycans containing a terminal fucose using biotinylated AAL. The shaded profile is the secondary antibody alone. C, flow cytometric analysis demonstrated equivalent binding of FITC-conjugated L-PHA to complex N-glycans in C1+/+ and C1−/− MEFs. The shaded profile is the unstained Slc35c1−/− MEFs. D, C1+/+ (white bars) and C1−/− (black bars) MEFs were transfected with TP-1 reporter and control plasmids and co-cultured with Delta1/L, Jagged1/L, or L cells. Notch signaling was determined as the ratio of firefly:Renilla luciferase activities. One representative experiment is shown. E, ligand-induced Notch signaling determined by fold-activation and normalized to 100% based on C1+/+. Error bars are S.E. (n = 3 independent experiments); *, p < 0.05 based on a one-tailed (Delta1/L); ***, p < 0.0001 based on a two-tailed (Jagged1/L) Student's t test.