FIGURE 3.

Slc35c2 inhibits some fucosylation. A, LEC11B cells stably expressing CHO Slc35c2 (C2; black line) or empty vector (Vec; gray line) were assayed for binding of mouse anti-SSEA-1 antibody by flow cytometry. The shaded profile is the secondary antibody alone. B and C, CHO cells transiently transfected with cDNAs encoding CHO Fut6B (B) or Fut9 (C) together with CHO Slc35c2 cDNA (C2; black line) or empty vector (Vec; gray line) were assayed for binding of mouse anti-SSEA-1 antibody by flow cytometry. The shaded profile is the secondary antibody alone. D, binding of FITC-conjugated PSA as in A. The shaded profile is unstained LEC11B cells.