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. Author manuscript; available in PMC: 2010 Nov 8.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Oct 1;2010:pdb.emo141. doi: 10.1101/pdb.emo141

TABLE 1.

Developmental Events in Aedes aegypti Embryogenesis (after Raminani and Cupp, 1978). The timing of major embryonic developmental events is indicated in hrs after egg laying (AEL) at 25° C.

Age
(hrs AEL)
Event
0.5 Polar body formation
1 Fertilization
1-2 1st and 2nd mitotic divisions
3 Formation of pole cells and primary yolk cells
4 Peripheral migration of cleavage nuclei
5 Pole cells outside the blastoderm
6 Syncitial blastoderm
8 Cellularization of blastoderm
9 Germ band formation; secondary yolk cells
11 Pole cell entry into the blastoderm
12 Initiation of gastrulation; Migration of presumptive mesodermal cells, elongation of the germ band
14 Amnion and serosa formation
15 Segmentation of the embryo begins; Ventral nerve cord formation begins.
18 Labral lobe formation
19 Anterior midgut-stomodaeal and posterior midgut-proctodaeal invaginations form.
25 Antennae, mandibles and maxillae begin to form; anterior migration of the mouthparts begins.
30 Vitelline membrane present; germ band retraction begins; labial lobes appear.
35 Blastokinesis and dorsal closure begin; foregut differentiation and formation of the midgut begins; formation of the brain and gonads initiates.
40 Differentiation of the suboesophageal ganglion and tracheal system starts.
45 Hindgut differentiation begins; formation of the retrocerebral complex and stomodaeal nervous system initiates.
50 Breakdown of the amnion and serosa; oenocytes and fat cells are formed; muscles of the head and mouthparts begins to form.
60 Differentiation of trunk musculature
65 Heart formation
75 Formation of the hatching spine begins.
96 Embryogenesis completed