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Plk2 levels are not increased by DNA damage under hypoxia. (A) H460 cells were treated with CPT-11 (100 μg/mL) for 22 hours. After the first 2.5 hours of CPT-11, cells were placed in 0.2% hypoxia for 19.5 hrs, harvested, run on a 7.5% SDS-PAGE gel and immunoblotted for Plk2 and Ran (loading control). (B) HCT 116 cells were treated with Adriamycin (0.2 μg/mL, 20 hrs). Cell lysates were run on a 7.5% SDS-PAGE gel and immunoblotted for Plk2 and β-actin (loading control).
