TABLE 1.
Pharyngeal torsion angles in animals with various mig-6 genotypes
| Strains | Minimum angle(°) | Maximum angle (°) | Mean angle ± SEM(°) | n |
|---|---|---|---|---|
| Wild type | ||||
| N2 | 0 | 0 | 0 | 20 |
| Homozygotes and heterozygotes of mig-6(et4) and mig-6(sa580) | ||||
| mig-6(et4) | 17.9 | 43.3 | 28.9 ± 1.2 | 20 |
| mig-6(sa580) | 13.9 | 33.3 | 23.8 ± 1.2 | 20 |
| mig-6(et4)/+ | 4.1 | 29 | 14 ± 1.2 | 20 |
| mig-6(sa580)/+ | 2.3 | 18 | 8.3 ± 1.1 | 20 |
| mig-6(et4)/mig-6(sa580) | 12.4 | 32.6 | 21.9 ± 1.1 | 20 |
| mig-6 alleles other than et4 and sa580 | ||||
| mig-6(k177) | 0 | 0 | 0 | 20 |
| mig-6(ev701) | 0 | 0 | 0 | 20 |
| mig-6(ev700) | 0 | 0 | 0 | 20 |
| mig-6(e1931) | 0 | 0 | 0 | 20 |
| mig-6(oz113) | 0 | 0 | 0 | 20 |
| mig-6(ev788)/eT1a | 0 | 0 | 0 | 20 |
| Rescue of mig-6(sa580) using cosmids ZK742 + C37C3 | ||||
| Line 1, ZK742 + C37C3 in mig-6(sa580) | 3.2 | 17.9 | 12.6 ± 1.0 | 17 |
| Line 2, ZK742 + C37C3 in mig-6(sa580) | 5.8 | 17.5 | 12.2 ± 1.3 | 11 |
| Line 3, ZK742 + C37C3 in mig-6(sa580) | 2.1 | 12.8 | 7.9 ± 0.6 | 25 |
| Line 4, ZK742 + C37C3 in mig-6(sa580) | 1.4 | 11.9 | 5.3 ± 0.6 | 27 |
| Line 5, ZK742 + C37C3 in mig-6(sa580) | 1.4 | 19.5 | 8.7 ± 1.9 | 11 |
| Rescue of mig-6(et4) using minigenes mig-6S and mig-6L | ||||
| Line 1, pZH125 (mig-6S) in mig-6(et4) | 0.1 | 16.1 | 3.8 ± 0.9 | 20 |
| Line 2, pZH125 (mig-6S) in mig-6(et4) | 0.1 | 23 | 8.2 ± 1.2 | 20 |
| Line 3, pZH125 (mig-6S) in mig-6(et4) | 0.9 | 25.5 | 8.1 ± 1.4 | 20 |
| Line 4, pZH125 (mig-6S) in mig-6(et4) | 0.2 | 25.5 | 6.3 ± 1.2 | 20 |
| Line 1, pZH117 (mig-6L) in mig-6(et4) | 0.0 | 29.5 | 11.9 ± 2.2 | 20 |
| Line 2, pZH117 (mig-6L) in mig-6(et4) | 2.2 | 28.1 | 14.1 ± 1.5 | 20 |
| Rescue of mig-6(sa580) using minigenes mig-6S and mig-6L | ||||
| Line 1, pZH125 (mig-6S) in mig-6(sa580) | 2.6 | 27.9 | 12.4 ± 1.9 | 20 |
| Line 2, pZH125 (mig-6S) in mig-6(sa580) | 0.9 | 25.9 | 11.2 ± 1.5 | 20 |
| Line 1, pZH117 (mig-6L) in mig-6(sa580) | 2.8 | 25.2 | 11.1 ± 1.9 | 11 |
| Rescue of mig-6(et4) using a mig-17∷GFP translational reporter | ||||
| Line 1, mig-17∷GFP in N2 | 0 | 0 | 0 | 20 |
| Line 1, mig-17∷GFP in mig-6(et4) | 5.2 | 21.7 | 12.5 ± 1.1 | 20 |
| Phenocopies of et4 and sa580 phenotypes in wild-type N2 | ||||
| Line 1, pQC10.1 (pZH125_et4) in N2 | 0.6 | 14.7 | 7.2 ± 1.0 | 20 |
| Line 2, pQC10.1 (pZH125_et4) in N2 | 4.5 | 21.9 | 12.8 ± 1.3 | 15 |
| Line 3, pQC10.1 (pZH125_et4) in N2 | 7.1 | 29 | 16.8 ± 1.7 | 15 |
| Line 1, pQC10.2 (pZH125_sa580) in N2 | 2.9 | 17.7 | 10.8 ± 0.9 | 22 |
a
ev788 is a putative null allele, and homozygotes are lethal; eT1 is used as a balancer.