TABLE 1.
List of plasmids
| Plasmid number | Description | Source |
|---|---|---|
| YIp1090 | LEU2, IME1(−1318 to +202)-lacZ | Kassir lab |
| YIp2102 | IME1UASru-HIS4TATA-LacZ, LEU2 | This work |
| YEp2536 | TRP1, 2μ, pCDC28-GST-MSN2 | Shenhar and Kassir (2001) |
| YEp2562 | 2μ, URA3, pCDC28-3xHA-sok2(1–32, 766–2721) | This work |
| YEp2780 | TRP1, 2μ, pADH1-GAL4(bd)-ime1(270–360) | Rubinstein et al. (2007) |
| YIp2887 | ime1(−3762 to −1118)-URA3-ime1(+945 to +2100) | This work |
| YIp2895 | sum1-URA3-sum1(Sum1 is from −49 to −1 and from +3189 to +3244, complete deletion of ORF) | This work |
| P2980 | IME1w/o IREu (IME1 is from −3.7 to +2.1) | This work |
| YCp3010 | IME1(−1283 to −1370)UASruab-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3021 | IME1(−1203 to −1370)UASru-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3022 | IME1(−789 to −915)UASrm-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3023 | IME1(−623 to −757)UASv-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3031 | UAShis4-IME1(−916 to −1122)URSd-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3032 | UAShis4-IME1(−1370 to −1643)UCS3-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3039 | UAShis4-IME1(−345 to −622)UCS1-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YCp3040 | UAShis4-2xIME1(−1171 to −1197)URSuE-GFP-ADH1t, pTEF-natR-tTEF, CEN6, ARSH4 | This work |
| YIp3085 | gzf3Δ∷hisG-URA3-hisG | This work |
| YIp3101 | yox1Δ∷hisG-URA3-hisG | This work |
| YIp3107 | rim101∷hisG-URA3-hisG | This work |
| YIp3125 | IME1(Sc)(−3.8 to +2.1), URA3 | This work |
| YIp3129 | IME1(Sp −2175 to −1, Sc −33 to +2100), URA3 | This work |
| YIp3130 | IME1(Sb −2119 to −1, Sc −33 to +2100), URA3 | This work |
| YIp3131 | sum1(+1782 to 3168)-6HA, k1TRP1 | This work |
| YIp3153 | sum1Δ∷URA3 | This work |
| YIp3198 | upc2Δ∷hisG-URA3-hisG | This work |
| YIp3199 | spt23Δ∷hisG-URA3-hisG | This work |
Sc, Saccharomyces cerevisiae; Sp, Saccharomyces paradoxus; Sb, Saccharomyces bayanus.