TABLE 2.
Yeast strains
| Strain | Relevant genotype | Source |
|---|---|---|
| BY4256 | MATα, hoΔ∷RPL39pr-tdTomato-hph, can1Δ∷STE2pr-SpHIS5, lyp1Δ, his3Δ1, leu2Δ0, ura3Δ0, met15Δ0 | Kainth et al. (2009) |
| Y8205 | MATα, hoΔ∷RPL39pr-tdTomato-hph, can1Δ∷STE2pr-SpHIS5, leu2Δ∷STE3pr-LEU2, lyp1Δ, his3Δ1, ura3Δ0, met15Δ0 | Kainth et al. (2009) |
| Y422 | MATa/MATα, ura3-52/ura3-52, trp1Δ/trp1Δ, leu2-3,112/leu2-3,112, ade2-1/ade2-R8 | Sagee et al. (1998) |
| Y1061 | MATα, ura3-52, trp1Δ, leu2-3,112, his3∷hisG-URA3-hisG, ade2-R8, gal80∷hisG | Parent of Y1065 |
| Y1064 | MATa, ura3-52, leu2,3-112, trp1Δ, his3∷hisG, ade2-1, gal80∷hisG, gal4∷hisG | Shenhar and Kassir (2001) |
| Y1065 | MATα, ura3-52, trp1Δ, leu2-3,112, his3∷hisG, ade2-R8, gal80∷hisG gal4∷hisG | Shenhar and Kassir (2001) |
| Y1075 | MATa, ura3-52, leu2,3-112, trp1Δ, his3∷hisG, ime1∷hisG | (Shenhar and Kassir 2001) Isogenic to Y1064 |
| Y1076 | MATα, ura3-52, leu2-3,112, trp1Δ, his3∷hisG, ime1∷hisG | (Shenhar and Kassir 2001) Isogenic to Y1065 |
| Y1162 | MATa, ura3-52, sok2Δ∷TRP1,trp1Δ, his3∷hisG | (Shenhar and Kassir 2001) Isogenic to Y1064 |
| Y1171 | MATa/MATα, ura3-52/ura3-52, trp1Δ/trp1Δ, his3∷hisG/his3∷hisG, msn2∷HIS3/msn2∷HIS3, msn4∷URA3/msn4∷URA3 | HO induced diploidization of Y1132 (Shenhar and Kassir 2001) |
| Y1214 | MATa, ura3-52, leu2,3-112∷LEU2-UASru-his4-lacZ, trp1Δ, his3∷hisG | Y1064 transformed with YIp2102 cut PpuMI |
| Y1631 | MATa/MATα, IME1/IME1 | Y1064 × Y1065 |
| Y1699 | MATα, IME1-ΔIREu | Isogenic to Y1065 |
| Y1721 | MATa/MATα, ura3-52/ura3-52, trp1Δ/trp1Δ, leu2-3,112/leu2,3-112∷LEU2-UASru-his4-lacZ, his3∷hisG/his3∷hisG-URA3-hisG, ade2-R8/ade2-1, gal80∷hisG/gal80∷hisG, gal4∷hisG/GAL4, met/MET | Y1214 × Y1061 |
| Y1794 | MATa, IME1-ΔIREu | Isogenic to Y1064 |
| Y1795 | MATa/MATα IME1-ΔIREu/IME1-ΔIREu | Y1794 × Y1699 |
| Y1802 | MATa, ura3-52∷URA3- IME1(−3.8 to +2.1), leu2,3-112, trp1Δ, his3∷hisG, ime1∷hisG | Y1075 transformed with YIp3125 cut StuI |
| Y1803 | MATα, ura3-52∷URA3- IME1(−3.8 to +2.1), trp1Δ, leu2-3,112, his3∷hisG, ime1∷hisG | Y1076 transformed with YIp3125 cut StuI |
| Y1804 | MATa, ura3-52∷URA3-IME1p-SP-Ime1-SC, leu2,3-112, trp1Δ, his3∷hisG, ime1∷hisG | Y1075 transformed with YIp3129 cut StuI |
| Y1805 | MATα, ura3-52∷URA3-IME1p-SP-Ime1-SC, trp1Δ, leu2-3,112, his3∷hisG, ime1∷hisG | Y1076 transformed with YIp3129 cut StuI |
| Y1806 | MATa, ura3-52∷URA3-IME1p-SB-Ime1-SC, leu2,3-112, trp1Δ, his3∷hisG, ime1∷hisG | Y1075 transformed with YIp3130 cut StuI |
| Y1807 | MATα, ura3-52∷URA3-IME1p-SB-Ime1-SC, trp1Δ, leu2-3,112, his3∷hisG,, ime1∷hisG | Y1076 transformed with YIp3130 cut StuI |
| Y1808 | MATa/MATα, ura3-52∷URA3- IME1(-3.8 to +2.1)/ura3-52∷URA3- IME1(-3.8 to +2.1), ime1∷hisG/ime1∷hisG | Y1802 × Y1803 |
| Y1809 | MATa/MATα, ura3-52∷URA3- IME1p-SP-Ime1-SC/ura3-52∷URA3-IME1p-SP-Ime1-SC, ime1∷hisG/ime1∷hisG | Y1804 × Y1805 |
| Y1810 | MATa/MATα, ura3-52∷URA3-IME1p-SB-Ime1-SC/ura3-52∷URA3-IME1p-SB-Ime1-SC, ime1∷hisG/ime1∷hisG | Y1806 × Y1807 |
| Y1824 | MATa, ura3-52, leu2,3-112∷LEU2-UASru-his4-lacZ, yox1Δ∷hisG-URA3-hisG, trp1Δ, his3∷hisG | Y1214 transformed with a 6.7-kb SacII fragment from YIp3101 |
| Y1825 | MATa, ura3-52, leu2,3-112∷LEU2-IME1-lacZ, trp1Δ, his3∷hisG, ade2-1, gal80∷hisG, gal4∷hisG | Y1064 transformed with YIp1090 cut PpuMI |
| Y1827 | MATa, sum1Δ, ura3-52, leu2,3-112∷LEU2-UASru-his4-lacZ, trp1Δ, his3∷hisG | Y1214 transformed with 1.6-kb EcoRI fragment from YIp3153 |
| Y1836 | MATa, ura3-52, leu2,3-112∷LEU2-IME1-lacZ, trp1Δ, gzf3Δ∷hisG-URA3-hisG, his3∷hisG, ade2-1, gal80∷hisG, gal4∷hisG | Y1825 transformed with 7.7-kb SpeI–XhoI fragment from YIp3085 |
| Y1837 | MATa, SUM1-6HA-kiTRP1-sum1, ura3-52, leu2,3-112∷LEU2-UASru-his4-lacZ, trp1Δ, his3∷hisG | Y1214 transformed with YIp3131 cut EcoNI |
| Y1854 | MATa, rim101Δ∷hisG-URA3-hisG, ura3-52, trp1Δ, leu2,3-112∷LEU2-UASru-his4-lacZ, his3∷hisG | Y1214 transformed with 4.8-kb NotI fragment from YIp3107 |
| Y1855 | MATa, swi4∷URA3, ura3-52, trp1Δ, his3∷hisG, leu2,3-112∷LEU2-UASru-his4-lacZ | Y1214 transformed with 1636 (from K. Nasmyth) cut SacI–SalI |
| Y1858 | MATa, tec1∷HIS3, ura3-52, trp1Δ, his3∷hisG, leu2,3-112∷LEU2-UASru-his4-lacZ | Y1214 transformed with p55217 (G. Fink) cut SacI–EcoRI |
| Y1859 | MATa, upc2∷ hisG-URA3-hisG, ura3-52, trp1Δ, his3∷hisG, leu2,3-112∷LEU2-UASru-his4-lacZ | Y1214 transformed with 6.0-kb SacI–SacII fragment from YIp3198 |
| Y1860 | MATa, spt23Δ∷hisG-URA3-hisG, ura3-52, trp1Δ, leu2,3-112∷LEU2-UASru-his4-lacZ, his3∷hisG | Y1214 transformed with 6.0-kb SacI–SacII fragment from YIp3107 |
| Y1877 | MATα, sum1Δ∷URA3, ura3-52, trp1Δ, leu2-3,112, his3∷hisG, ade2-R8, gal80∷hisG, gal4∷hisG | Y1065 transformed with 1.6-kb EcoRI fragment from YIp3153 |
| Y1891 | MATa/MATα, sum1Δ∷URA3/sum1Δ | Y1827 × Y1877 |