Figure 1.—

Temporal progression of wound closure in control larvae. (A–E) Dissected whole mounts of larvae heterozygous for the e22c-Gal4 driver and UAS-DsRed2-Nuc. Nuclei expressing DsRed2-Nuc are red and membranes immunostained for Fasciclin III are green. (A) Unwounded epidermal sheet. (B–E) Epidermal sheet after wounding: (B) ∼5 min post wounding, (C) 4 hr post wounding, (D) 8 hr post wounding, and (E) 24 hr post wounding. Note that the epidermal sheet is closed. Solid arrowheads, wound-edge cells that retain Fasciclin III staining facing the wound gap; open arrowheads, wound-edge cells that lack Fasciclin III staining facing the wound gap; arrows, elongated epidermal cells; dots, multinucleate epidermal cells. Bar, 100 μm.