Figure 4.—

UAS-RNAi transgenes affecting wound closure show distinct wound morphologies. (A–I) Dissected whole mounts of larvae heterozygous for e22c-Gal4,UAS-DsRed2-Nuc (red) and the indicated UAS-RNAi transgene were immunostained for Fasciclin III (green) 24 hr post wounding. UAS-RNAi transgenes were grouped into seven classes on the basis of wound morphology. (A) Class I, bsk^x2; (B) class I, DFos/kay; (C) class I, DJun/Jra; (D) class II, hep^x2; (E) class III, SCAR; (F) class IV, Ced-12; (G) class V, mbc; (H) class VI, Rac1; (I) class VII, msn^x2. See results for classification criteria. Stars indicate disorganized epidermal sheet; arrowheads indicate smooth wound edge with pronounced fluorescence; brackets span zones of nuclear crowding. Lines with two UAS-RNAi inserts targeting the same gene are denoted by ^x2. Bar, 100 μm.