Figure 6.—

JNK activation in larvae expressing UAS-RNAi transgenes affecting wound closure. (A–L) Dissected epidermal whole mounts of unwounded (A and J) or pinch wounded (B–I and K and L) larvae heterozygous for the e22c-Gal4 driver (A–K) or the A58-Gal4 driver (L), the JNK activity reporter msn-lacZ (A–L), and the indicated UAS-RNAi transgene. All whole mounts were stained 6 hr post wounding or post mock wounding with X-Gal to detect β-galactosidase reporter activity (blue). (A) w¹¹¹⁸, unwounded; (B) w¹¹¹⁸, wounded; (C) bsk^x2; (D) DJun/Jra; (E) DFos/kay; (F) hep,Mkk4; (G) SCAR; (H) Ced-12; (I) mbc; (J) Rac1, unwounded; (K) Rac1, wounded; (L) msn^x2. Morphological classes are indicated above the panels. Bar, 100 μm.