Figure 6.—

Quantitative PCR analysis of Veju retrotransposons in parental lines and in the S1–S5 generations of the derived allohexaploid. In addition, the natural hexaploid T. aestivum (cv. Chinese spring–CS) was used for comparison. (A) LTR:internal Veju sequence. (B) Relative quantity (RQ) of the Veju internal region (sequence). (C) RQ of the Veju LTR. Shaded bars represent the observed relative quantity (mean ± SE, n = 3), while hatched bars represent the expected relative quantity (see text for qPCR validation).