Figure 2.—

Genetic interactions among AMR and septin mutations. (A–D) Synthetic lethality between cdc4-s16 and myp2Δ. (A) Strains JW400 (cdc4-s16) and TP5 (myp2Δ) were crossed, and tetrads were dissected and incubated on YE5S medium at 32° for 6 days. Nine tetrads were tetratypes (i.e., contained one wild-type spore, two single-mutant spores, and one inviable or very slow-growing double-mutant spore) and one was a parental ditype (i.e., each spore was a single mutant and thus grew well). (B–D) DIC micrographs of aberrant cytokinesis in strains JW400, TP5, and JW345 (cdc4-s16 myp2Δ). Cells were grown overnight in YE5S liquid medium at 30°. Bar (B–D), 10 μm. (E and F) Absence of synthetic interaction between myo2-E1 myp2Δ and spn1Δ. (E) Strains TP90 (myo2-E1 myp2Δ) and JW380 (myp2Δ spn1Δ) were crossed, and tetrads were dissected and incubated on a YE5S plate at 25° for 7 days. Circles, myo2-E1 myp2Δ segregants; squares, myo2-E1 myp2Δ spn1Δ segregants. (F) Cells of strains TP90 and JW2560 were grown exponentially in YE5S liquid medium at 25°, shifted to 36° for 4 hr, and examined. Bar, 10 μm.