TABLE 1.

S. pombe strains used in this study

Strain	Genotypea	Source/reference
972	h− wild type	Leupold (1970)
DM1274	h− scw1Δ∷ura4+ ade6-M210 leu1-32 ura4-D18	Jin and McCollum (2003)
FY435	h+ ade6-M210 his7-366 leu1-32 ura4-D18	S. Forsburg
FY11065	h− cds1-2HA-6His∷ura4+ leu1 ura4	Yeast Genetic Resource Center Japan
KS3320	h+ swi5Δ∷kanMX6	Anders et al. (2008)
KS3250	h− rec12Δ∷kanMX6 ade6-M210 his7-366 leu1-32 lys1-37	Anders et al. (2008)
MBY580	h− bgs1-191 ade6-M210 leu1-32 lys1-131 ura4-D18	Liuet al. (1999)b
TP5	h− myp2Δ∷his7+ ade6-M210 his7-366 leu1-32 ura4-D18	Bezanilla et al. (1997)
TP90	h+ myo2-E1 myp2-Δ1∷his7+ade6-M216 his7-366 leu1-32 ura4-D18	M. Bezanilla and T. Pollard
YDM74	h− myo2-E1 ade6 his3-D1 leu1-32 ura4-D18	D. McCollumc
JW21	h+ cdc4-8	P. Nurse; Nurse et al. (1976); McCollum et al. (1995)
JW81	h− ade6-M210 leu1-32 ura4-D18	Lab collection
JW182	h− spn4-Δ2	This studyd
JW183	h− spn4+-GFP	This studyd
JW215	h+ his3-27 leu1-32 ura4-D18	Lab collection
JW249	h− spn1-Δ2	This studyd
JW251	h− spn1+-GFP	This studyd
JW267	h− 81nmt1-spn1+ 81nmt1-spn4+ leu1-32 ura4-D18	See text.
JW289	h+ spn1-Δ2 leu1-32 ura4-D18	JW215 × JW249
JW290	h− spn1-Δ2 his3-27 ura4-D18	JW215 × JW249
JW295	h+ spn4-Δ2 leu1-32 ura4-D18	JW215 × JW182
JW306	h+ spn1+-GFP his3-27 leu1-32 ura4-D18	JW215 × JW251
JW314	spn4-Δ2 mid2Δ∷ura4+ leu1-32 ura4-D18	JW295 × JW430
JW315	scw1-ng124 mid2Δ∷ura4+ leu1-32 ura4-D18	ng124e × JW430
JW318	spn1+-GFP mid2Δ∷ura4+ leu1-32 ura4-D18	JW306 × JW430
JW320	scw1-ng124 spn4+-GFP leu1-32 ura4-D18	JW331 × JW183
JW321-1	scw1-ng124 spn1-Δ2 ura4-D18	JW331 × JW290
JW326	h− mid2+-GFP ade6-M210 leu1-32 ura4-D18	See text.
JW330	h− scw1-ng124 ade6-M210 leu1-32 ura4-D18	This studyf
JW331	h+ scw1-ng124 ade6-M210 leu1-32 ura4-D18	This studyf
JW332	mid2+-GFP spn4-Δ2 leu1-32 ura4-D18	JW326 × JW295
JW345	cdc4-s16 myp2Δ∷his7+ ade6-M210 his7-366 leu1-32 ura4-D18	JW400 × TP5
JW374	art1-s34 spn1-Δ2 leu1-32 ura4-D18	JW403 × JW289
JW380	h− myp2Δ∷his7+ spn1-Δ2 ade6 his7-366 leu1-32 ura4-D18	This studyg
JW396	h− s26 leu1-32 ura4-D18	This studyh
JW397	h− s63 his3-27 leu1-32 ura4-D18	This studyh
JW398	h+ s28 leu1-32 ura4-D18	This studyh
JW399	h− cdc4-s16 leu1-32 ura4-D18	This studye
JW400	h+ cdc4-s16 ade6-M210 his7-366 leu1-32 ura4-D18	JW399 × FY435
JW401	cdc4-s16 spn4-Δ2 leu1-32 ura4-D18	JW399 × JW295
JW402	h− art1-s34 81nmt1-spn1+ 81nmt1-spn4+ leu1-32 ura4-D18	This studyi
JW403	h− art1-s34 leu1-32 ura4-D18	This studye
JW404	h+art1-s34 leu1-32 ura4-D18	This studye
JW405	h− rgf3-s44 81nmt1-spn1+ 81nmt1-spn4+ leu1-32 ura4-D18	This studyi
JW406	h− rgf3-s44 his3-27 leu1-32 ura4-D18	This studye
JW407	h+ rgf3-s44 his3-27 leu1-32 ura4-D18	This studye
JW409	h−rgf1-GFP ade6-M210 leu1-32 ura4-D18	See text.
JW412	h−rgf3-GFP ade6-M210 leu1-32 ura4-D18	See text.
JW423	h− rgf1-Δ1 ade6 leu1-32 ura4-D18	This studyj
JW430	h− mid2Δ∷ura4+ade6 leu1-32 ura4-D18	D. Brunner and P. Nurse
JW729	h+ ade6-M210 leu1-32 ura4-D18	Lab collection
JW903	h+ 3nmt1-rgf2 ade6-M210 leu1-32 ura4-D18	See text.
JW1078	h− 3nmt1-rgf1 ade6-M210 leu1-32 ura4-D18	See text.
JW1081	h− 3nmt1-rgf3 ade6-M210 leu1-32 ura4-D18	See text.
JW1084	h− sad1-mRFP1 ade6-M210 leu1-32 ura4-D18	See text.
JW1100	h+ spn1-mEGFP ade6-M210 leu1-32 ura4-D18	See text.
JW1105	h+ rgf3-mEGFP ade6-M210 leu1-32 ura4-D18	See text.
JW1113	h− spn1-mEGFP sad1-mRFP1 ade6-M210 leu1-32 ura4-D18	JW1100 × JW1084
JW1122	3nmt1-rgf3 spn1-mEGFP ade6-M210 leu1-32 ura4-D18	JW1081 × JW1100
JW1123	3nmt1-rgf1 spn1-mEGFP ade6-M210 leu1-32 ura4-D18	JW1078 × JW1100
JW1124	rgf1-GFP sad1-mRFP1 ade6-M210 leu1-32 ura4-D18	This studyk
JW1125	rgf3-s44 spn1-mEGFP leu1-32 ura4-D18	JW406 × JW1100
JW1126	art1-s34 spn1-mEGFP ade6-M210 leu1-32 ura4-D18	JW403 × JW1100
JW1128	rgf3-GFP spn1-Δ2 ade6-M210 leu1-32 ura4-D18	JW412 × JW289
JW1131	rgf3-mEGFP sad1-mRFP1 ade6-M210 leu1-32 ura4-D18	JW1105 × JW1084
JW1139	rgf1-GFP spn1-Δ2 ade6-M210 leu1-32 ura4-D18	JW409 × JW289
JW1203	mid2-GFP scw1-ng124 ade6-M210 leu1-32 ura4-D18	JW326 × JW331
JW2068	h+ sec15Δ∷kanMX4 ade6 leu1-32 ura4-D18	Lab collection
JW2155	h− scw1-ng124 ade6-M210 leu1-32 ura4-D18	This studyl
JW2271	h+ scw1-ng124 ade6-M216 leu1-32 ura4-D18	This studyl
JW2286	scw1Δ∷ura4+ spn1-Δ2 ade6-M210 leu1-32 ura4-D18	DM1274 × JW289
JW2499	cdc4-s16 scw1-ng124 ade6 leu1-32 ura4-D18	JW399 × JW2271
JW2503	myo2-E1 scw1-ng124 ade6 leu1-32 ura4-D18	YDM74 × JW2271
JW2560	myo2-E1 myp2-Δ1∷his7+ spn1-Δ2 ade6 his7-366 leu1-32 ura4-D18	This studym
JW2613	h+/h− scw1-ng124/scw1+ ade6-M216/ade6-M210 leu1-32/leu1-32 ura4-D18/ura4-D18	JW2271 × JW81
JW2614	h+/h− scw1-ng124/scw1Δ∷ura4+ ade6-M216/ade6-M210 leu1-32/leu1-32 ura4-D18/ura4-D18	DM1274 × JW2271

^aAll deletions and taggings constructed in this study by the PCR method used the kanMX6 marker, which is not indicated in the genotypes. The mating types of some strains were not determined. GFP(S65T) is indicated simply as GFP; mEGFP (see materials and methods) is indicated as such.

^bbgs1-191 was originally known as drc1-191 (Liu et al. 1999).

^cmyo2-E1 was originally referred to as rng5-E1 (Balasubramanian et al. 1998; Eng et al. 1998).

^dStrain 972 was transformed with the appropriate constructs (see text).

^eSegregant from the third backcross of the original mutant isolate to strains JW81 and JW215.

^fSegregant from the fourth backcross of the original mutant isolate to strains JW81 and JW215.

^gDerived by several crosses from strains TP5 and JW249.

^hSegregant from the first backcross of the original mutant isolate to strain JW215.

ⁱThe original mutant isolate.

^jSegregant from a heterozygous mutant obtained after PCR-mediated transformation (see text) of diploid strain JW9 (Wu et al. 2001).

^kDerived by crossing JW409 to a segregant from the cross of JW1084 to JW729 (Wu et al. 2003).

^lSegregant from the 10th backcross of the original mutant isolate to strains JW81 and JW729.

^mDerived by several crosses from strains TP5, YDM74, and JW249.